Table 1. Data Collection Statistics for AdoMetDC Mutants and Complexes^a.

	D174N (−Put)	E256Q (−Put)	E178Q (−Put)	D174N (−Put)/MeAdoMet	E256Q (−Put)/MeAdoMet	E178Q (−Put)/MeAdoMet	AdoMetDC (−Put)	E178Q (+Put)	E256Q (+Put)
wavelength	0.9764	0.9764	0.9766	0.9795	0.9795	0.9795	1.5418	0.9792	0.9792
space group (Å)	C2	C2	C2	C2	C2	C2	C2	C2	C2
a (Å)	90.60	90.71	90.57	99.22	94.44	100.15	90.57	91.60	92.35
b (Å)	55.24	55.90	56.26	50.60	50.08	51.07	55.54	54.49	53.54
c (Å)	74.22	74.33	74.05	68.82	70.17	68.95	74.31	74.58	74.71
β (°)	109.64	109.84	109.73	104.95	105.00	105.40	109.66	109.45	109.09
resolution (Å)	1.84	1.88	1.98	1.70	2.00	1.97	2.35	2.10	1.75
total reflections	88255	71106	53478	122776	54248	73956	41261	69216	123289
unique reflections	27642	27282	21433	35113	18180	23589	14524	19713	34072
redundancy	3.2(2.8)	2.6(2.3)	2.5(2.2)	3.5(2.5)	2.9(2.7)	3.1(2.5)	2.8(2.6)	3.5(2.8)	3.6(3.0)
% completeness	92.1(73.8)	96.7(87.0)	89.8(89.4)	96.3(78.0)	84.0(72.6)	98.6(91.1)	99.1(95.7)	96.8(79.8)	97.7(89.6)
I/σ	15.9(2.2)	16.0(2.0)	14.4(2.0)	15.4(2.2)	9.9(3.3)	15.0(3.4)	13.9(2.0)	34.4(6.2)	30.1(4.6)
Rsymb	6.4(39.0)	4.9(39.6)	7.1(43.8)	8.3(32.8)	10.7(25.6)	8.0(23.8)	9.1(46.8)	3.7(12.2)	4.2(15.8)
Matthewʼs coef. (Å3/Da)	2.28	2.31	2.32	2.18	2.09	2.22	2.30	2.29	2.28
solvent content (%)	45.1	45.9	45.9	42.5	40.1	43.5	45.5	45.3	45.0

^aThe values in parenthesis are for the highest resolution shell.

^bR_sym>=ΣΣ_i|I_i − ⟨I⟩|/Σ⟨I⟩, where ⟨I⟩ is the mean intensity of the N reflections with intensities I_i and common indices h,k,l.