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Downstream signaling pathways in tumors from COX-2 transgenic and wild type mice. Paraffin-embedded tumors from the mid-colon of age-matched COX-2 transgenic and wild type mice were immunostained to detect EGFR, pEGFR, Akt, and pAkt. Wild type and transgenic tissue was processed together. We found similar results with antibodies specific for phospho-Tyr992-EGFR and phospho-Thr308-Akt.
