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Assessment of EGFR and Akt signaling pathways in normal appearing tissue from COX-2 transgenic and wild type mice. Paraffin-embedded tissue samples from the mid-colon of age-matched COX-2 transgenic and wild type mice were immunostained to detect EGFR, pEGFR, Akt, and pAkt. Wild type and transgenic tissue was processed together. Similar results were obtained with antibodies to detect phospho-Tyr992-EGFR and phospho-Thr308-Akt.
