Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Agarose gel analysis of the pMS2 constructs. Lanes 1 and 8 show pMS2 DNA, whereas lane 2 shows the same after digestion with EcoRV. Lanes 3 and 4 represent the construct before and after the removal of the scaffold, respectively, of a mock ligation mixture, which did not contain a dodecamer. Lanes 5−7 show pMS2 constructs containing control, T[5-Me,8]G, and G[8,5-Me]T dodecamer, respectively, after enzymatic removal of the scaffold 58-mer.
