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. 2009 Jan 13;2:8. doi: 10.1186/1756-0500-2-8

Figure 1.

Figure 1

GAPDH mRNA and protein expression under extreme hypoxic (0.1% O2), normoxia and reoxygenation conditions in HepG2, Hep 1-6, Hep-3-B, A-549, HT-29 and HCT-116 cells, in vitro. (A) Semiquantitative RT-PCR analysis of GAPDH mRNA expression in HepG2, Hep 1-6 and Hep-3-B (B) Western blot expression analysis of GAPDH protein under identical conditions in HepG2, Hep 1-6 and Hep-3-B. (C) Semiquantitative RT-PCR analysis of GAPDH mRNA expression in A-549, HT-29 and HCT-116, in vitro (D) Western blot analysis of GAPDH protein expression under identical conditions in A-549, HT-29 and HCT-116. Representative experiments out of three for each experimental set.