Figure 6.
LS174T colon carcinoma cells tether on an E-selectin (5 μg/mL) patterned glass slide. The slide was uniformly functionalized with E-selectin on half its area and with discrete patches of immobilized E-selectin surrounded by inert PEG-functionalized regions on the other half. (a) and (b) are scanning electron microscopy images of the photoresist templates of the two regions, respectively, on the same slide, taken at least 200 μm away from all the edges. The scale bar represents 40 μm. (a) LS174T cells were perfused through uniform E-selectin (c) and patches of E-selectin (d) at 0.70 dyn/cm2. (b) LS174T cells were perfused over uniform E-selectin (e) and patches of E-selectin (f) at 0.35 dyn/cm2. The red arrows indicate the transient cell binding to E-selectin patches. The scale bar represents 100 μm.