Figure 2.

Viscosity dependence of k_fast in stopped-flow during mismatched dNTP incorporation by WT Pol β and I260Q. Reactions were initiated by rapid mixing of equal volumes of solution A (containing 1 μM Pol β and 400 nM 19/36AP(T)/15) and solution B (containing 4 mM dGTP). Reactions were performed at 37 °C in Pol β assay buffer at pH 8.5 containing 5 mM free Mg²⁺ and differential glycerol in both syringes.