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. 2008 Aug 22;47(37):9718–9727. doi: 10.1021/bi800689d

Figure 3.

Figure 3

Pol β T:G mismatch titration at pH 8.5. Reactions were initiated by rapid mixing of equal volumes of solution A (containing 1 μM Pol β and 400 nM 19/36AP(T)/15) and solution B (containing varied dGTP concentration). Reactions were performed at 37 °C in Pol β assay buffer containing 5 mM free Mg2+. (A) A hyperbolic fit of the fast phase rate (kfast) versus dGTP concentration (after mixing) yields a k2 of 255 ± 9 s−1, a Kd of 433 ± 72 μM, and a y0 of 148 ± 13 s−1. (B) A hyperbolic fit of the slow phase rate (kslow) versus dGTP concentration (after mixing) yields a Kd,app of 489 ± 26 μM and a kpol of 5.7 ± 0.1 s−1.