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. 2008 Aug 22;47(37):9718–9727. doi: 10.1021/bi800689d

Figure 5.

Figure 5

I260Q correct dNTP incorporation in stopped-flow. I260Q T:A matched incorporation into 19/36AP(T)/15 (teal trace). Reactions were initiated by rapid mixing of equal volumes of solution A (containing 1 μM Pol β and 400 nM DNA substrate) and solution B (600 μM dATP) at pH 8.5. The slow phase fits to a rate of 48.5 ± 0.4 s−1, while rapid quench overlay (open circles) fits to a rate of 47.6 ± 2.5 s−1. The inset shows the corresponding T:A match reaction using dideoxy-terminated primer (gray trace).