Figure 6.

Viscosity dependence of k_fast and k_slow in stopped-flow during correct dNTP incorporation by WT Pol β versus I260Q. Reactions were initiated by rapid mixing of equal volumes of solution A (containing 1 μM Pol β or I260Q and 400 nM 19/36AP(T)/15) and solution B (containing 600 μM dATP). Reactions were performed at 37 °C in Pol β assay buffer at pH 7.6 containing 5 mM free Mg²⁺ and differential glycerol in both syringes.