Figure 7.

Comparison of WT Pol β and I260Q T:G mismatched incorporation at pH 8.5. Reactions were initiated by rapid mixing of equal volumes of solution A (containing 1 μM Pol β or I260Q and 400 nM 19/36AP(T)/15) and solution B (containing 4 mM dGTP) and performed at 37 °C in Pol β assay buffer at pH 8.5 containing 5 mM free Mg²⁺ and 10% glycerol. Fast and slow phase misincorporation rates were 323 ± 6 s⁻¹ and 4.5 ± 0.01 s⁻¹ correspondingly for WT (red trace), and 383 ± 6 s⁻¹ and 12.2 ± 0.03 s⁻¹ correspondingly for I260Q (blue trace).