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LC-MS/MS SRM chromatogram of renal venous perfusate obtained from untreated, isolated, and perfused SHR kidney. Two transitions were monitored: 278 → 141 m/z for the internal standard (top), which was 13C10-adenosine; and 330 → 136 m/z for endogenous 3′,5′-cAMP (bottom). Note the prominent peak with a retention time of approximately 2.9 min (bottom), which was much too short to be 3′,5′-cAMP, which has a retention time of approximately 6.3 min.
