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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Mol Cell Neurosci. 2008 Dec 14;40(3):338–343. doi: 10.1016/j.mcn.2008.11.005

Figure 2. The production of Eag80 is activity-dependent.

Figure 2

COS cells were transfected with Eag alone (A, C-E) or co-transfected with PKA or PKC (B). Cell lysates were separated by SDS-PAGE and Eag detected by immunoblot. TPA (162 nM, A) and ionophore (10 μM, C) can induce the production of Eag80 protein. (B) Cotransfection with PKA or PKC induces Eag80 and occludes TPA induction. Both effects are blocked by bisindolylmalemide I (BisI), an inhibitor of PKC (at low concentration) and PKA (at high concentration). (D) BisI blocks TPA-induced production of Eag80 in cells transfected with Eag alone. (E) MAPK inhibitor inhibits the production of Eag80 in cells transfected with Eag alone. Kinase inhibitors, at indicated concentrations, were added ca. 23 h after transfection, TPA was added 1 h later and cells harvested after an additional 5 hours.