Table 3.1.
Studies of macromolecule diffusion through mucus and other porous medium
| Set up | Medium | Author | Summary |
|---|---|---|---|
| Chamber | Native and purified mucus | Bhat et al.[113] | small drugs diffusion in native and purified porcine gastric mucus |
| Broghton et al.[72] | 200 nm fluorescent nanospheres diffusion through respiratory mucus | ||
| Desai et al.[76, 92] | diffusion of radioactive solutes of various MW and charge | ||
| Mucin | Norris et al.[77] | fluorescent polystyrene particles with functional groups, diameter 124-560 nm | |
| Polymer | Kosto et al.[101, 102] | fluorescent conjugated Ficoll diffusion in composite dextran/agarose membrane | |
| Diffusion through gel | Native, purified, or artificial mucus | Radomsky et al.[79] | protein and antibody diffusion in capillary tube by fluoresence microscopy |
| Olmsted et al.[31] | antibody virus and macro (polymeric and viral) particles fluorescent with FRP and multispeed particle tracking (MIP) | ||
| Saltzman et al.[12] | antibody and proteins in a capillary system using FRAP and epi-fluoresence microscopy | ||
| Shen et al.[52] | measurement of fluorescent DNA molecule by FRAP | ||
| Park et al.[85] | holographic spectrometry of BSA migration in polyacrylamide gels | ||
| Larhed et al.[13] | diffusion of radioactive solute in artificial mucus | ||
| Lai et al.[30] | high-speed tracking of surface modified fluorescent polymer particles in fresh cervical mucus | ||
| Lafitte et al.[89, 90] | diffusion of probes and PEG in non-commercial purified porcine gastric mucus with NMR | ||
| Dawson et al.[73] | diffusion of fluorescent 100-500 nm polystyrene by rapid particle tracking | ||
| Polymer | Amsden et al.[84] | albumin diffusion alginate gel quantitation | |
| Agarose | Berk et al.[81] | protein (MW 14-600kDa) and Dextran diffusion in agarose and cell mixture by FRP | |
| Dykstra et al.[114] | radioactive small drugs in agarose | ||
| Gustafsson et al.[115] | ethanol by holographic laser interferon | ||
| Pluen et al.[50] | of globular and flexible molecules by FRAP | ||
| Gosnell et al.[51] | tracking of DNA migration by UV dye in agarose gels | ||
| Dextran | Kosar et al.[40] | tracking diffusion of protein in Dextran solution by holographic interferometry | |
| Actin | Liedl et al.[82] | tracking of quantum dots in actin solution with FCS | |
| Commercial fibrous membrane | Gibbs et al.[88] | NMR detection of protein movement through membrane | |
| Bohrer et al.[100] | diffusion of flexible polymers (Dextran and Ficoll) detection by FCS | ||
| Comparative studies of medium | Henry et al.[83] | Epi-fuorescence imaging of solute through agar, carbopol and mucus | |
| Johnson et al.[20, 80] | FRP analysis of protein and Ficoll | ||
| Larhed et al.[93] | diffusion of small radioactive solutes in native and purified gastric mucus | ||
| Other studies | in vivo | Szentkuti et al.[95] | delivery of luminal dye, fluorescent Dextran and polystyrene beads 14-500 nm to rat colon |
| Combined diffusion and epithelial cell uptake | Tolo et al.[116] | diffusion of radioactive125I-albumin through rabbit oral and colon mucus epithelial | |
| Osth et al.[94] | uptake of fluorescent ovalbumin coated starch microparticles by pig respiratory mucus and cell layer |