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. 2009 Mar 2;4(3):e4658. doi: 10.1371/journal.pone.0004658

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Yin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Time course of transglycosylation reaction of wildtype Endo-A (squares) and the three mutants, N171A (circles), Y205F (triangles), and Y299F (diamonds). The reactions were carried out using a chitinase-coupled assay, in which Man₉GlcNAc₂Asn-Fmoc was used as the donor substrate and 4MU-GlcNAc as the acceptor, 0.25 µg of each enzyme was used in a 50 µl system.