Table 1. Hydrolysis and transglycosylation activities of Endo-A mutants.

Mutant	Specific hydrolysis activitya	Percentage of specific hydrolysis activity	Specific transglycosylation activitya	Percentage of specific transglycosylation activity
	mg min−1 mg−1	%	µmol min−1 mg−1	%
Wildtype	21.1	100	1.72	100
N171A	NDb	-	0.01	0.53
Y205F	9.39	44	1.61	94
Y299F	21.4	101	5.11	297

^aThe hydrolysis activity was determined using Ribonuclease B as the substrate, the transglycosylation activity was measured using a chitinase-coupled assay, in which Man₉GlcNAc₂Asn-Fmoc was used as the donor substrate and 4MU-GlcNAc as the acceptor.

^bND, not detected.