Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Dec 3;28(49):13258–13267. doi: 10.1523/JNEUROSCI.3109-08.2008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008 Society for Neuroscience 0270-6474/08/2813258-10$15.00/0

PMC Copyright notice

Figure 4. — A fraction of PrP in the brains of Tg(WT-E3) mice is insoluble and can be immunoprecipitated by 15B3 antibody. A, Brain lysates prepared from mice of the following genotypes and ages were subjected to ultracentrifugation at 186,000 × g for 40 min, and PrP in the supernatants (S lanes) and pellets (P lanes) was analyzed by Western blotting using 6D11 antibody: non-Tg/Prnp^+/+, 156 d (lanes 1–2); Tg(WT-E3^+/−)/Prnp^0/0, 162 d (lanes 3–4); Tg(WT-E3^+/+)/Prnp^0/0, 162 d (lanes 5–6); Tg(PG14^+/−)/Prnp^0/0, 156 d (lanes 7–8). B, Supernatant and pellet fractions obtained in B were subjected to immunoprecipitation using the monoclonal antibody 15B3. Immunoprecipitated PrP was analyzed by Western blotting with antibody 8H4.