Table 3.

Summary of T cell-DC contact measurements of published lymph node datasets (Bousso & Robey, 2004), and thymocyte-DC contacts. For the percent of time points in contact with DC, the discordance rate with manual analysis is computed by the absolute difference between automated result and manual measurement Automated results were found to agree with unpublished manual measurements (Ladi et al., 2008).

Dataset Type	# of Datasets	# of Thymocytes	Time points with DC contact	Total Time Points examined	Percent of time points in contact with DC	Discordance with manual analysis	# of DC contacts	Total time of tracks examined (hours)	Frequency of contacts per hour	Avg. duration of contact (min)
T-cell without antigen	1	51	124	548	22.63%	N/A	28	4.57	6.13	2.36
T-cell with antigen	1	31	314	437	71.85%	N/A	28	3.64	10.44	5.70
Wild Type	4	402	844	6270	13.46%	1.46%	184	63.9	2.88	2.79
(P14 → B6)	6	456	1808	5354	33.77%	7.73%	328	54.6	6.01	3.47
P14 → (DBA × B6)	3	219	753	3037	24.79%	0.49%	140	31.0	4.52	3.51