Table 3. Probability of Interlaboratory and Intercytokine Differences in Recovery of Cytokines across Assays.
| interlaboratory |
|||||||
|---|---|---|---|---|---|---|---|
| IL-1β |
IL-6 |
intercytokine |
|||||
| assay | 86/552 | 86/680 | assay calibrators | 89/548 | assay calibrators | IL-1β | IL-6 |
| BioSource | <0.0001a | NA | <0.0001 | 0.280 | 0.007 | <0.0001 | 0.005 |
| Endogen | NAb | NA | NA | <0.0001 | 0.023 | <0.0001 | <0.0001 |
| Quantikine | <0.0001 | <0.0001 | 0.018 | 0.054 | <0.0001 | <0.0001 | <0.0001 |
| QuantiGlo | <0.0001 | 0.003 | <0.0001 | 0.465 | 0.002 | <0.0001 | <0.0001 |
| Beadlyte Luminex | 0.552 | 0.001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| in-house Luminex | <0.0001 | <0.0001 | 0.014 | <0.0001 | 0.199 | 0.054 | <0.0001 |
| MSD Small Spot | 0.094 | 0.049 | 0.028 | 0.814 | 0.049 | <0.0001 | <0.0001 |
P < 0.01 indicates that the % recovery was different between laboratories or between cytokines (Mann−Whitney, Kruskal−Wallis).
NA = not applicable; the analysis was not applicable when <2 laboratories provided data from the same kit.