Table 1. Peptides from Known Neuropeptide Precursorsa.
| precursor | sequence | ESI MS (m/z) | obs. mass (Da) | error (ppm) | LC-ESI-FTMS | LC-MALDI-TOF/TOF | LC-ESI ion trap | LC-MALDI-TOF MS |
|---|---|---|---|---|---|---|---|---|
| Arg-vasopressin[24−32] | A.C*YFQNC*PRGamide.G | 1083.49 | −42.5 | X | ||||
| Provasospressin[24−35] | A.CYFQNCPRGGKR.A | 1427.67 | −7.0 | X | ||||
| Provasopressin[157−168] | T.QESVDSAKPRVY | 1377.59 | 65.3 | X | ||||
| Provasopressin[156−168] | G.TQESVDSAKPRVY | 1478.69 | 33.8 | X | ||||
| Provasopressin[155−168] | A.GTQESVDSAKPRVY | 1535.69 | 39.1 | X | ||||
| Provasopressin[158−168] | Q.ESVDSAKPRVY | 1249.59 | 32.0 | X | ||||
| Provasopressin[154−168] | L.AGTQESVDSAKPRVY | 1606.69 | 62.2 | X | ||||
| Provasopressin[151−168] | L.VQLAGTQESVDSAKPRVY | 1947.09 | −41.1 | X | ||||
| Provasopressin[160−168] | S.VDSAKPRVY | 1033.56 | −9.7 | X | ||||
| Provasopressin[36−43] | R.ATSDMELR.Q | 921.47 | −54.3 | X | ||||
| CART[28−36] | A.pQEDAELQPR.A | 534.68 | 1067.52 | −26.2 | X | X | ||
| CART[82−86] | R.IPIYE.K | 633.34 | 633.34 | 4.7 | X | X | ||
| TRH[178−199] | R.FIDPELQRSWEEKEGEGVLMPE.K | 873.40 | 2617.24 | 0.0 | X | X | X | |
| Proenkephalin A[100−104] | R.YGGFM.K | 573.23 | −10.5 | X | ||||
| Proenkephalin A[198−209] | R.SPQLEDEAKELQ.K | 693.80 | 1385.67 | −7.2 | X | X | ||
| Proenkephalin A[198−209] | R.(Ac)SPQLEDEAKELQ.K | 1427.67 | 7.0 | X | ||||
| Proenkephalin A[219−229] | R.VGRPEWWMDYQ.K | 1465.65 | −6.1 | X | X | |||
| Proenkephalin A[114−133] | K.MDELYPVEPEEEANGGEILA.K | 735.10 | 2203.56 | 190.6 | X | X | ||
| Proenkephalin A[219−238] | R.VGRPEWWMDYQKRYGGFLKR.F | 2571.30 | 0.0 | X | ||||
| Protachykinin 1[58−68] | R.RPKPQQFFGLMGamide.K | 1347.70 | 5.2 | X | ||||
| Protachykinin 1[98−110] | R.HKTDSFVGLMGKR.A | 1474.74 | 20.3 | X | ||||
| VIP[111−124] | R.ISSSISEDPVPVKR.Q | 1512.73 | 52.9 | X | ||||
| VIP[156−169] | R.SSEGDSPDFLEELE.K | 1552.75 | −70.8 | X | ||||
| POMC[103−120] | R.AEEETAGGDGRPEPSPREamide.G | 941.97 | 1881.90 | −31.9 | X | X | ||
| POMCI[124−136] | R.SYSMEHFRWGKPVamide.G | 811.80 | 1621.76 | 12.3 | X | |||
| POMC[124−137] | R.(Ac)SYSMEHFRWGKPVG.K | 1721.91 | −63.9 | X | ||||
| Somatostatin[89−100] | R.SANSNPAMAPRE.R | 622.70 | 1243.50 | 47.4 | X | X | X | |
| Somatostatin[91−100] | A.NSNPAMAPRE.R | 543.80 | 1085.60 | −101.3 | X | |||
| Somatostatin[103−116] | K.AGCKNFFWKTFTSC | 1638.80 | −42.7 | X | ||||
| Secretogranin 1[585−594] | R.SFAKAPHLDL.K | 549.73 | 1097.44 | 132.1 | X | X | ||
| Secretogranin 1[597−611] | R.pQYDDGVAELDQLLHY.R | 879.00 | 1760.87 | −45.4 | X | X | ||
| Secretogranin 1[435−451] | R.LLDEGHDPVHESPVDTA.K | 915.90 | 1829.82 | 10.9 | X | X | X | |
| Secretogranin 1[49−61] | S.SAPTITPECRQVL.R | 707.80 | 1413.70 | 21.2 | X | |||
| Secretogranin 1[47−56] | S.KSSAPTITPE.C | 514.25 | 1028.45 | 97.2 | X | |||
| Secretogranin 1[21−34] | S.APVDNRDHNEEMVT.R | 1625.74 | −18.5 | X | ||||
| Secretogranin 1[383−395] | R.NHPDSELESTANR.H | 1468.73 | −54.5 | X | ||||
| Secretogranin 1[383−394] | R.NHPDSELESTAN.R | 1312.48 | 53.3 | X | ||||
| Secretogranin 1[572−584] | R.PFSEDVNWGYEKR.S | 1625.74 | 6.2 | X | ||||
| Secretogranin 2[571−583] | R.IPAGSLKNEDTPN.R | 678.22 | 1354.67 | 0.0 | X | |||
| Secretogranin 2[529−568] | R.VPSPGSSEDDLQEEEQLEQAIKEHLGQGSSQEMEKLAKVS.K | 4366.08 | −2.3 | X | ||||
| Secretogranin 2[287−297] | R.(Ac)SGHLGLPDEGN.R | 1136.49 | 17.6 | X | ||||
| Secretogranin 3[23−36] | A.FPKPEGSQDKSLHN.R | 792.30 | 1582.78 | −6.3 | X | |||
| Secretogranin 5[198−210] | K.SVPHFSEEEKEPE | 1542.79 | −71.3 | X | ||||
| Secretogranin 5[198−210] | K.(Ac)SVPHFSEEEKEPE | 1584.84 | −94.7 | X | ||||
| Secretogranin 5[180−195] | R.SVNPYLQGKRLDNVVA.K | 1771.87 | 50.8 | X | ||||
| Secretogranin 5[180−195] | R.(Ac)SVNPYLQGKRLDNVVAKK.S | 2070.23 | −33.8 | X | ||||
| Secretogranin 5[177−189] | R.KRRSVNPYLQGKR.L | 1600.83 | 62.5 | X | ||||
| Proenkephalin B[235−247] | R.SQENPNTYSEDLDV | 806.09 | 1609.61 | 37.3 | X | |||
| Proenkephalin B[211−218] | R.PKLKWDNQ.K | 514.79 | 1027.56 | −9.7 | X | |||
| Proenkephalin B[47−59] | C.SLECQDLVPPSEE.W | 722.21 | 722.21 | 13.8 | X | |||
| Proenkephalin B[166−174] | R.YGGFLRKYP.K | 1099.60 | 1099.60 | −18.2 | X | |||
| Proenkephalin B[221−234] | R.YGGFLRRQFKVVTR.S | 1725.98 | 1725.98 | −46.4 | X | |||
| Proenkephalin B[228−248] | R.pQFKVVTRSQENPNTYSEDLDV | 2451.61 | 2451.61 | −24.5 | X | |||
| Cortistatin[20−33] | L.LLLSGIAASALPLE.S | 1366.71 | 1366.71 | 65.8 | X | X | ||
| Somatotropin[208−216] | R.RFAESSCAF | 1016.49 | −49.2 | X | ||||
| ProSAAS[245−260] | R.LENSSPQAPARRLLPP. | 1744.37 | 136.4 | X | ||||
| ProSAAS[245−260] | R.LENSSPQAPARRLLPP.(+Na) | 1766.37 | 132.2 | X | ||||
| ProSAAS[42−59] | R.SLSAASAPLAETSTPLRL.R | 892.89 | 1783.97 | 0.6 | X | X | X | |
| ProSAAS[42−58] | R.SLSAASAPLAETSTPLR.L | 1670.80 | 47.9 | X | ||||
| ProSAAS[42−57] | R.SLSAASAPLAETSTPL.R | 757.29 | 1514.58 | 132.0 | X | |||
| ProSAAS[221−242] | R.AVDQDLGPEVPPENVLGALLRV.K | 2300.20 | 13.5 | X | ||||
| ProSAAS[206−218] | G.SSEPEAAPAPRRL.R | 1379.53 | 132.6 | X | ||||
| ProSAAS[62 −75] | R.AVPRGEAAGAVQEL.A | 1366.54 | 131.7 | X | ||||
| ProSAAS[45- 59] | S.AASAPLAETSTPLRL.R | 1496.68 | 93.5 | X | ||||
| ProSAAS[44- 59] | L.SAASAPLAETSTPLRL.R | 1583.71 | 88.4 | X | ||||
| Neurotensin[150−164] | R.pQLYENKPRRPYILKR.A | 1955.99 | 56.2 | X | ||||
| Neurotensin[150−162] | R.pQLYENKPRRPYIL.A | 1671.91 | 0.0 | X | ||||
| PACAP[51−68] | R.GMGENLAAAA VDDRAPLT.K | 1770.90 | −22.6 | X | ||||
| Progonadoliberin I[24−33] | S.pQHWSYGLRPGamide.G | 1181.31 | 111.6 | X | ||||
| Progonadoliberin I[24−33]+Na | S.pQHWSYGL RPGamide.G(+Na) | 1204.52 | 33.2 | X | ||||
| ProMCH[131−143] | R.EIGDEENSAKFPIamide.G | 724.73 | 1446.45 | 160.4 | X | X | ||
| Interleukin-3[54−62] | K.LPVSGLNNS.D | 899.39 | 33.4 | X |
a
Period (.) indicates cleavage sites. Bold type indicates putative novel peptides. “X” indicates type of MS instrument used to identify peptides. Tandem MS data was obtained in all cases except for the last column, indicating MALDI TOF data. Asterik (*) indicates the Cys−Cys bonds.