Table 2. Other Peptides and Proteins Identifieda.
| precursor | sequence | ESI MS (m/z) | obs. mass (Da) | error (ppm) | LC-ESI-FTMS | LC-MALDI TOF/TOF | LC-ESI ion trap | LC-MALDI-TOF MS |
|---|---|---|---|---|---|---|---|---|
| MBP S[2−18] | M.(Ac)ASQKRPSQR HGSKYLATA.S | 2026.99 | 34.0 | X | ||||
| MBP S[2−16] | M.(Ac)ASQKRPSQRHGSKYLA.S | 1854.90 | 45.8 | X | ||||
| MBP S[2−11] | M.(Ac)ASQKRPSQRHG.S | 1292.59 | 61.9 | X | ||||
| MBP S[183−195] | G.GRDSRSGSPMARR | 1431.69 | 14.0 | X | ||||
| DBI[2−14] | M.(Ac)SQADFDKAAEEVK.R | 1478.70 | −6.8 | X | ||||
| PEBP[9−25] | W.AGPLSLQEVDEPPQHAL.R | 1800.03 | −72.2 | X | ||||
| Cholecalcin[33−44] | K.LLIQSEFPSLL.K | 1258.74 | 1258.74 | −15.1 | X | X | ||
| T-kininogen I[378−385] | S.RPPGFSPF.R | 903.44 | 903.44 | 18.8 | X | X | ||
| Thymosin beta-10[2−44] | M.ADKPDMGEIASFDKAKLKK TETQEKNTLPTKETIEQEKRSEIS | 4932.52 | −2.0 | X | ||||
| Thymosin beta-4[2−44] | M.SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES | 4960.49 | −2.9 | X | ||||
| Ubiquitin[1−76] | MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG | 8559.62 | −1.2 | X | ||||
| Tubulin beta-5 chain[1−7] | MREIVHI.Q | 896.03 | 896.03 | 110.9 | X | X | ||
| Cullin 5-AVP-activated calcium-mobilizing receptorVACM-1 | D.KVPGGIEP.M | 795.45 | 5.0 | X |
a
Period (.) indicates cleavage sites. Bold type indicates putative novel peptides. “X” indicates type of MS instrument used to identify peptides. Tandem MS data was obtained in all cases except for the last column, indicating MALDI TOF data.