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. 2008 Jul 15;47(29):7715–7725. doi: 10.1021/bi8003704

Table 4. Steady-State Kinetic Constants for Hydrolysis of C6-HCTL by Dicobalt AHL Lactonase Variants.

enzyme KM (mM) kcat (s−1) kcat/KM (M−1 s−1)
wild typea 6.7 ± 0.7 198 ± 8 3.0 × 104
Y194F 0.6 ± 0.1 5.4 ± 0.2 9.0 × 103
D108N 1.3 ± 0.2 1.11 ± 0.04 8.5 × 102
a

Values taken from ref (7).