Table 5. Intrinsic Binding Energy of Ligands for the Dinucleotide Sitea.
NAD+ (mM) |
β-Me-TAD (μM) |
Tz (mM) |
ADP (mM) |
||||||
---|---|---|---|---|---|---|---|---|---|
enzyme | Kc | Kii | Kintr | Ki | Kintr | Ki | Kintr | Ki | Kintr |
TfIMPDHb | 140 | 6.8 ± 1.8 | 0.07 | 2.3 ± 0.4 | 0.02 | 50 ± 10 | 0.3 | 31 ± 2 | 0.2 |
CpIMPDHc | 4 | 4.9 ± 0.5 | 0.30 | 0.6 ± 0.04 | 0.1 | 1.5 ± 0.1 | 0.3 | 42 ± 6 | 8 |
hIMPDH2d | ≤0.2 | 0.59 ± 0.02 | 0.59 | 0.06 ± 0.02 | 0.06 | 1.3 ± 0.1 | 1.3 | 8.8 ± 2.2 | 8.8 |
The intrinsic binding constant for NAD+ is derived from the global fits. The intrinsic binding constants for β-Me-TAD, tiazofurin (Tz), and ADP are calculated from Kintr = Ki (1/(1 + Kc)).
See refs 14 and 23.
See ref 16.
See ref 24. The intrinsic binding constant for NAD+ is derived from the global fits.