Table I.
Real-time PCR primer and probe sequences used to validate microarray expression
| Genea | NCBI Accession Number | Forward Primer (5′–3′) | Probe (5′–3′) 6FAM-Sequence-MGBNFQ | Reverse Primer (5′–3′) |
|---|---|---|---|---|
| MHC1 | U84789 | CAGATAGATAAGGCGGGAGCTACT | CAGTGACAGTTCCC | GCCGTGAGAGACACATCAGAAC |
| SFRP4 | NM_001032962 | CTGCGAGCCCCTCATGAA | CACAGCTGGCCCGAA | GTCATAGACGGGCAGCTCATC |
| CCL3 | BV209119 | GGCCTGCTGTAGGCAGTCAT | CAGACTGACCAATGTGTATC | ACAGCCCTGACCAAAAGCAT |
| CAT | NM_001752 | TCCATTCGATCTCACCAAGGT | CAA GGACTACCCTCTCAT | CCGGTTTAAGACCAGTTTACCAA |
| StAR | BV208869 | GTGCGTGTGTACAAGGCAGAA | CGGCAGCCCTCTG | GGTGTTGCTGTGCAGTGAATG |
| ADAMTS-5 | XM_001103439 | TTCATGTTTCCCTCAGATAGTGATG | ACACATGTCATAGAAACAA | TGGTTTATTTGCTCTAAGCCCTCTA |
| MMP-9 | XM_001104871 | AGGCGCTCATGTACCCTATGTAC | CCCCCCTTGCATAAG | TGCCGGATGCCATTCAC |
| MMP-19 | XM_001111542 | TGGCCCCCAACTCCATT | TGTCTTAGACAGCCCTTC | ACCATTGACCTCCAGAAAAGAGAT |
| CASP2 | NM_032982 | GCACTGAGGGAGACCAAGCA | TCACCACCCTTTCCGGGCTTCAG | CACAGCTCAACGGTGGGAGTA |
| IL1RN | U65590 | TGGCACTTGGAGACGTGTATG | CCTCGGCCTGTCTC | CGAGTCTTTTTTCCTGCTCTGAA |
| AHRIP | NM_003977 | TCCAAAAACGTGTGATACAGGAA | CTCCCGGACTTTC | TAGTGGAACGTGGCCTTGGT |
| ARNT2 | BV208380 | CCAGTCCCAGGGAATGAATG | CTCCCGGCAGCACT | CACGTTCTCGCATAGCTTATGG |
| VEGF | M27281 X15997 | ACGAAGTGGTGAAGTTCATGGA | TCAGCGCAGCTACT | CTCATCAGGGTACTCCTGGAAGA |
| PRLR | NM_000949 | AGCTGAGTGGGAGACCCATTT | AAACAGAGTTTAAGATTCTC | GCGAACCTGGACAAGGTATTTC |
| CRHBP | NM_001882 | GACAGACCCCAACCTCTTTCC | CCCTGGTCGTTCCAC | TGGAGAAGCTGCAGTTTCGA |
| INHBA | BV166389 | AGAGCACCTGGCACGTCTTC | CAGCATCCAGCGGTT | ACGTCCAGGGAGCTTTTTCC |
| SC4MOL | AB169840 | TGGAGTGTAGCGGCACAATC | CGGCTCACTGCAAC | GGAGGCTGAGGCAGGAGAAT |
| LOC693394 | XM_001081982 | CACGTCATCGACTACATCTTGGA | CCTGGACTCGCATCC | TCTGGTGATGGAGGCTGACA |
| ESR2 | AB006589 | TCAGTTGAGGTCAGGAGTTTGAAA | CAACATGGCGAAACC | ACCACCATGCCTGGCTAATT |
| AR | NM_001032911 | GCAGGCAAGAGCACTGAAGATA | TGCTGAGTATTCCCC | CCTTTGGTGTAACCTCCCTTGA |
| PGR | XM_001095317 | CATGTCAGTGGGCAGATGCT | TATTTTGCACCTGATCTAA | ATTCTTTCATCCGCTGTTCATTC |
| PGRMC1 | XM_001100639 | CCAGGACCCCCGCATAC | CATGGCCATCAACG | TTTGGTCACGTCGAACACCTT |
aBased on NCBI Entrez Gene Terminology.