Table IV.

Real-time PCR validation of select differentially expressed genes

		Microarray					Real-time PCR
	Gene	Control (C) Microarray relative expression	Antide (A)	A+LH	A+LH+TRL	A+LH+TRL+R5020	One-Way ANOVA; P	Similar to Microarray Pattern
Not Regulated	AR	65 ± 5	≡C	≡C	≡C	≡C	>0.9	Yes
	CASP2	16 ± 1	≡C	≡C	≡C	≡C	>0.2	Yes
	ESR2	23 ± 2	≡C	≡C	≡C	≡C	>0.6	Yes
	PGR	11 ± 1	≡C	≡C	≡C	≡C	>0.6	Yes
LH Regulated	AHRIP	124 ± 12	↓	↑	≡LH	≡LH	<0.01	No
	IL1RN	23 ± 4	↑	↓	≡LH	≡LH	<0.001	Yes
LH and Progestin Regulated	ARNT2	39 ± 5	↑	↓	↑	↓	<0.01	Yes
	CRHBP	75 ± 30	↑	↓	↑	trend ↓	<0.001	Yes
	LOC693394	26 ± 9	trend ↑	↓	↑	↓	<0.03	Yes
	SFRP4	30 ± 5	↑	↓	↑	↓	<0.02	Yes
	SC4MOL	2774 ± 369	↓	↑	↓	↑	<0.02	Yes
	StAR	8370 ± 569	↓	↑	↓	↑	<0.001	Yes
LH and Other Steroid Regulated	ADAMTS5	24 ± 5	↓	↑	↓	≡TRL	<0.01	Yes
	CAT	2803 ± 445	↓	↑	↓	≡TRL	<0.05	Yes
	Inhibin BA	679 ± 138	↓	↑	↓	≡TRL	>0.3	Yes
	PRLR	87 ± 18	↓	↑	↓	≡TRL	<0.01	Yes
	VEGF	282 ± 26	↓	↑	↓	≡TRL	<0.05	Yes
	CCL3	133 ± 64	↑	↓	↑	≡TRL	>0.06	Yes
	MHC1	383 ± 232	↑	trend ↓	↑	≡TRL	<0.01	No
	MMP19	132 ± 18	↑	↓	↑	≡TRL	<0.05	Yes
	MMP9	116 ± 35	↑	↓	trend ↑	≡TRL	<0.05	No
	PGRMC1	2109 ± 82	↑	↓	↑	≡TRL	<0.01	No

Control values (±SEM) are presented as a reference. Direction of expression of validated genes by microarray analysis is compared with previous group (e.g. A compared with C), except for genes whose expression did not differ from control. One-way ANOVA values of significance are presented for real-time PCR validation.