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The absorbance decay of the semiquinones at 450 nm as a function of time in native and the mutant DN(M17) and DN(L210) RCs in the presence of 1 mM CdSO4. This represents the rate of proton transfer in RCs with a bound Cd2+ metal ion. Note that kAB(2) is decreased ≈10-fold in the mutant RCs compared with native RCs. The observed decay rate for the two mutant RCs is the same within experimental error. Conditions: same as in Fig. 1.
