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. 2008 Dec 22;37(3):849–857. doi: 10.1093/nar/gkn1003

Figure 6.

Figure 6.

UV-visible results of calf-thymus DNA and its Chol (A), DDAB (B), DOPE (C) and DOTAP (D) complexes: spectra of (a) free DNA (125 μM); (b) free lipid (40 μM); (1–8) lipid–DNA complexes 1 (5 μM), 2 (10 μM), 3 (15 μM), 4 (20 μM), 5 (25 μM), 6 (30 μM), 7 (35 μM) and 8 (40 μM). Plot of 1/(A – A0) versus (1/drug concentration) for lipid and calf-thymus DNA complexes, where A0 is the initial absorbance of DNA (260 nm) and A is the recorded absorbance (260 nm) at different lipid concentrations (5–40 μM) with constant DNA concentration of 125 μM at pH 7.4 for Chol (A′), DDAB (B′), DOPE (C′) and DOTAP(D′).