. 2008 Dec 22;37(3):877–890. doi: 10.1093/nar/gkn1007

Table 1.

I-AniI variants identified by a bacteria-based cleavage assay

I-AniI variants	Survival rate (%)^a
Wild type	<0.2
F13Y	36 ± 3.8
F13L, K46Q, S92T	75 ± 9.6
F13Y, S111Y (Y2)	95 ± 30
F13Y, I55V	85 ± 16
F13Y, K46Q, S92T	93 ± 4.7
F13V, K46Q, S92T	3.5 ± 1.2
F13Y, S111F, K222R	94 ± 18
F13L, K46Q, F91I	71 ± 11
L4I, K49Q, S111F, V209A	90 ± 17
F13L, S92T^b	68 ± 11
F13L, K46Q^b	2.7 ± 2.4
S111Y^b	2.6 ± 4.2
S111F^b	0.26 ± 0.28
F13Y, K222R^b	37 ± 13
L4I, K49Q, V209A^b	<0.2
F13Y, I55V, F91I, S92T, S111Y (M5)^b	92 ± 10

^aThe survival rate was determined by dividing the number of colonies on the selective plates by that on the nonselective plates. Errors refer to mean ± S.D. of three replicate plating experiments.

^bThese variants were created by site-directed mutagenesis. Boldface correspond to mutations (alone and in combination) found in the Y2 variant of I-AniI.