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. 2009 Jan 6;37(3):983–998. doi: 10.1093/nar/gkn1010

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — DNA bending by C.PvuII. WT C-boxes (A) and O_L spacer variant with CAA (B). With the indicated restriction enzymes 150-nt DNA fragments (60-ng each) were released, and contain the C-box in the center (EcoRV) or close to either end (MluI or BamHI). C.PvuII (200 ng) was added and EMSA was carried out (as in Figure 3). (C) Flexure displacement analysis was as described (52). The net DNA bending average angle was calculated.