Figure 7.

Mapping translational start for pvuIIM. Overlapping C-box sequences of pvuIIC and pvuIIM N-terminus in complementary strands shown at top. RBS, ribosome binding site. The E. coli RBS Logo (62) is aligned to M2 as initiator. The methionine residues are marked in bold. Presence of 4 WT methionine codons was designated as MMMM. Mutation of the first M codon to L is listed as LMMM; and so on. Each of the variants was translationally fused to lacZ. For all mutants, translation activity was measured as modified Miller units of LacZ as described in ‘Materials and methods’ section.