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Verification of isothermal strand-displacement polymerization reaction. The mixture containing 5.0 × 10−8 M probe and 5.0 × 10–8 M primer with 6.25 × 10–9 M target (curve a), 6.25 × 10–9 M random DNA (curve c) added at t0 in the presence of dNTPs/polymerase respectively; curve b: no target added.
