Skip to main content
. 2009 Jan 7;37(3):e20. doi: 10.1093/nar/gkn1024

Figure 6.

Figure 6.

Detection of different concentrations of target based on isothermal strand-displacement polymerization reaction. Experiments were performed in the presence of 15U polymerase Klenow fragment exo− and 100 μM dNTPs with 5 × 10–8 M probe, 5 × 10–8 M primer, and different concentrations of target. (A) Monitoring the fluorescence intensity of this amplified DNA detection method over a range of target DNA concentrations. The curves from a to i contain the target with 1.0 × 10–10, 2.0 × 10–11, 4.0 × 10–12, 8.0 × 10–13, 1.6 × 10–13, 3.2 × 10–14, 6.4 × 10–15, 1.28 × 10–15 and 0 M, respectively. All samples were incubated at 37°C. (B) The relationship of the rate of fluorescence enhancement with target DNA concentration.