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Changes in the absorption (left) and emission (right) spectra of 3HC-NC(11–55) on binding to SL2 RNA. The spectra of 0.4 µM 3HC-NC(11–55) was recorded in the absence (black) and in the presence of 0.2 (green) 0.4 (blue) and 0.6 µM (red) SL2 RNA in 10 mM phosphate buffer, 100 mM NaCl, pH 7.0. Excitation wavelength was 340 nm.
