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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: FEBS Lett. 2008 Dec 29;583(2):425–429. doi: 10.1016/j.febslet.2008.12.041

Figure 1. Comparison of [3H]-uridine uptake, [3H]-NBMPR binding, and mRNA expression between CCRF-CEM cells and Ara-C/8C cells.

Figure 1

A) 3x105 CCRF-CEM or Ara-C/8C cells (CEM-AraC-8C) cells were incubated with [3H]-uridine in sodium-free transport buffer for 5 min in the presence or absence of 1.0 μM NBMPR, a selective inhibitor of hENT1, as described in Materials and Methods. Data points represent sample mean +/− SD in duplicate (N=4; *p<0.01 different from –NBMPR, #p<0.01 different from parental cell line). B) [3H]-NBMPR binding was performed as described in Materials and Methods. Data points represent mean +/− SD from samples run in triplicate (N=2; *p<0.01). C) RT-PCR analysis of hENT1 mRNA expression was determined as described in Materials and Methods. Representative gel comparing mRNA levels in the parental CCRF-CEM cell line and CCRF-CEM-AraC-8C cell line.