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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: FEBS Lett. 2008 Dec 29;583(2):425–429. doi: 10.1016/j.febslet.2008.12.041

Figure 2. Substitution of G24 in hENT1 disrupts nucleoside and Ara-C transport activity.

Figure 2

A) Cross-species comparison of amino acids 13-33 of ENT1 and ENT2. Arrow denotes G24. B) Wt hENT1 and G24A, G24E, and G24R hENT1 mutants were expressed separately in PK15 cells (6×104) and [3H]-uridine uptake was performed for 20, 40, and 60 minutes as described in Materials and Methods. Average transport by wt hENT1 was approx. 4.19 pmol/min/mg protein. Data points represent mean +/− SD of samples run in quadruplicate (N=1; *p<0.01 different from control). C) Wt hENT1 and G24A, G24E, and G24R hENT1 mutants were expressed separately in PK15 cells (6×104) and [3H]-Ara-C uptake was performed for 2 hours in the presence or absence of 1μM NBMPR as described in Materials and Methods. Average transport by wt hENT1 was approx. 2.08 pmol/min/mg protein. Data points represent mean +/− SD of samples run in quadruplicate (N=1; *p<0.01 different from control, #p<0.01 different from –NBMPR).