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Size exclusion chromatography of apo-MnSOD. A thermostatted column of BioGel P-100 (1.5×100 cm) equilibrated with 20 mM MOPS (pH 7.5) containing 1 mM EDTA at 45°C was loaded with apo-MnSOD (trace A), Fe2-MnSOD (E170A) (trace B); or a MW calibration mixture (trace C: (1) bovine serum albumin; (2) Mn2-MnSOD; (3) carbonic anhydrase).
