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. Author manuscript; available in PMC: 2009 Nov 4.
Published in final edited form as: Biochemistry. 2008 Oct 9;47(44):11625–11636. doi: 10.1021/bi8015636

Figure 6.

Figure 6

Representative metal uptake timecourses for apo-MnSOD variants. Co2+ was added to apo-MnSOD (50 μg/mL in 20 mM MOPS) at t = 0 and binding was monitored fluorimetrically as described in the Methods. Reaction timecourses for mutational variants are presented as a stack plot. Four distinct kinetic patterns are distinguished: (A) biphasic, indistinguishable from WT; (B) biphasic, distinct from WT; (C) monophasic, fast-reacting; (D) monophasic, slow-reacting. Individual traces: (1) WT apo-MnSOD, 37°C, pH 7.6; (2) apo-MnSOD (R72C/D174C/bBm), 37°C, pH 7.6; (3) apo-MnSOD (H17C/N190C) (-SS-), 37°C, pH 7.6; (4) apo-MnSOD H30A, 33°C, pH 7.0; (5) apo-MnSOD Y174F, 37°C, pH 7.6; (6) apo-MnSOD Y34A, 31°C, pH 7.0; (7) apo-MnSOD Y34F, 37°C, pH 7.6; (8) apo-MnSOD S126C (-SS-), 37°C, pH 8.8; (9) apo-MnSOD S126C (-SH), 25°C, pH 7.6; (10) apo-MnSOD (S126C/Y174C) 2×(-SS-), 37°C, pH 7.6; (11) apo-MnSOD E170A, 37°C, pH 7.0; (12) apo-MnSOD E170C (-SS-), 37°C, pH 7.6;; (13) apo-MnSOD Y174C (-SS-), 37°C, pH 7.6; (14) apo-MnSOD H171A, 37°C, pH 7.0.