Figure 1.
Live/dead staining of biofilms grown at different depths. S. mutans UA159 was grown at different biofilm depths in BHI, stained with LIVE/DEAD BacLight fluorescent dye and analyzed with CLSM. The panels show images of 100-microns, 200-microns and 400-microns depths biofilms cultivated in the CDFF. Dead cells are stained red, and live cells are stained green. The images are representative of three biological experiments.