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. 2008 Dec 19;9:620. doi: 10.1186/1471-2164-9-620

Table 5.

Accession numbers and SSRs for GenBank deposited sequences http://www.ncbi.nlm.nih.gov/ amplified from 16 Phytophthora species (Table 1) using primers designed on P. infestans with the first approach (Fig. 1A).

Phytophthora
species 		SELECTED PRIMERS(a)
I3F-4R I5F-I6R I7F-I8R I9F-I10R I11F-I12R I13F-I14R
P. alni subsp. alni
SCRP2		 NS++
58°C/1.7 mM*		 EF216535
No SSR;
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216513
(aag)4
(agg)6
58°C/1.0 mM*		 NA+ EF216477
(agg)4
(aag)5
58°C/1.7 mM*
P. cambivora
SCRP67		 NS++
58°C/1.7 mM*		 NA+ NS++
58°C/1.7 mM*		 EF216516
(acg)4
(aag)5
(agg)6
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216478
(agg)5
(aag)5
58°C
1.7 mM*
P. cinnamomi
SCRP115		 NS++
58°C/1.7 mM*		 NS++
58°C/1.7 mM*		 NS++
58°C/1.7 mM*		 EF216509
(aag)14
58°C/1.0 mM*		 EF216494
(aag)4
58°C/1.7 mM*		 NS++
58°C/1.7 mM*
P. citricola
SCRP130		 NS++
58°C/1.7 mM*		 EF216534
(agc)4; (agc)5
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216520
(aag)4
58°C/1.0 mM*		 EF216498
(aag)4
58°C/1.7 mM*		 EF216482
(agg)9
(aag)5
58°C/1.7 mM
P. europaea
SCRP622		 NS++
58°C/1.7 mM*		 NS++
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216512
No SSR
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216476
(agg)5
(aag)5
58°C/1.7 mM*
P. fragariae
var. rubi
SCRP333		 NS++
58°C/1.7 mM*		 NA+ NA+ EF216500
(acg)4
58°C/1.0 mM*		 NA+ NA+
P. ilicis
SCRP377		 NS++
58°C/1.7 mM*		 EF216532
(agc)9
58°C/1.0 mM*		 NA+ EF216501
No SSR
58°C/1.0 mM*		 EF216495
No SSR
58°C/1.7 mM*		 EF216483
(aag)4
(agc)4
58°C/1.7 mM
P. infestans
sc 03.26.3.3		 NS++
58°C
1.7 mM*		 EF216524
(agc)6
(agc)5
58°C/1.0 mM		 NS++
58°C/1.7 mM*		 EF216499
(aag)11
58°C/1.0 mM*		 EF216487
(acc)8
58°C/1.7 mM*		 EF216474
(aag)7
(aag)4
58°C/1.7 mM
P. inundata
SCRP644		 NS++
58°C/1.7 mM*		 NS++
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216508
No SSR
58°C/1.0 mM*		 EF216497
No SSR
58°C/1.7 mM*		 NA+
P. lateralis
SCRP390		 NS++
58°C/1.7 mM*		 EF216527
(agc)4
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216507
No SSR
58°C/1.0 mM*		 EF216493
No SSR
58°C/1.7 mM*		 EF216481
(agg)5
(aag)4
58°C/1.7 mM*
P. nemorosa
SCRP910		 NS++
58°C/1.7 mM*		 EF216531
(agc)7
58°C/1.0 mM*		 NA+ EF216503
No SSR
58°C/1.0 mM*		 EF216492
(aag)4
58°C/1.7 mM*		 EF216486
(acg)4
(aag)4
(agc)4
58°C/1.7 mM*
P. pseudosyringae
SCRP674		 NS++
58°C/1.7 mM*		 EF216529
(agc)7
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216502
No SSR
58°C/1.0 mM*		 EF216496
(aag)5
58°C/1.7 mM*		 EF216485
(acg)4
(aag)4
58°C/1.7 mM*
P. psychrophila
SCRP630		 NS++
58°C/1.7 mM*		 EF216528
(agc)4
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 NA+ EF216491
(aag)4
58°C/1.7 mM*		 EF216484
(aag)4
(agc)4
58°C/1.7 mM*
P. quercina
SCRP541		 NS++
58°C/1.7 mM*		 NS++
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216506
No SSR
58°C/1.0 mM*		 EF216490
(aag)5
58°C/1.7 mM*		 EF216480
(agg)6
(aag)4
58°C/1.7 mM*
P. ramorum
SCRP911		 NS++
58°C/1.7 mM*		 EF216525
No SSR
58°C/1.0 mM*		 NS++
58°C/1.7 mM*		 EF216505
No SSR
58°C/1.0 mM*		 EF216489
(acc)4
58°C/1.7 mM*		 EF216479
(aac)7
(agg)9
58°C/1.7 mM
P. sojae
SCRP555		 NS++
58°C/1.7 mM*		 EF216526
No SSR
58°C/1.0 mM*		 NA+ EF216504
(agc)4
58°C/1.0 mM*		 EF216488
No SSR
58°C/1.7 mM*		 EF216475
(agg)7
(aag)5
58°C/1.7 mM*

(a) = Primers listed in Table 2 and not reported in this table are those that did not produce any reliable sequence (see text).

NA+ = Isolate-primer combinations that did not produce any amplification or produced complex profiles (two or more fragments).

NS++ = Isolate-primer combinations for which single PCR bands were obtained but direct sequencing did not produce reliable results.

°C/mM* = Selected annealing temperature (°C) and MgCl2 concentration (mM) in PCR reactions.