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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Pancreas. 2009 Mar;38(2):197–202. doi: 10.1097/MPA.0b013e31818d14a8

Figure 5. Effect of TRH on apoptosis of hIPCs as monitored by release of mitochondrial cytochrome C into the cytoplasm.

Figure 5

Uninfected hIPCs (A) or hIPCs infected with 3,000 particles of AdCMVmTRHR per cell (B) were incubated in serum-free medium without (A) or with 1 µM TRH (B) for 24 h and then fixed and stained for f-actin, cytochrome C and nuclei. TRHR-expressing cells not treated with TRH (not shown) were similar to uninfected hIPCs (A) demonstrating punctate cytochrome C staining indicating mitochondrial localization. TRHR-expressing hIPCs treated with TRH (B) showed dispersed cytoplasmic staining indicating leakage of cytochrome C from mitochondria.