TABLE 2.
Sequences of siRNA used in the infection
| Target genesa | Sequences of siRNAb | Target sites on mRNA |
|---|---|---|
| Smurf1 (NM 029438) | 5′–GATTCGAACCTTGCAAAGAAAGAC ttcaagagaGTCTTTCTTTGCAAGGTTCTTTTTTC–3′ |
372–390 |
| Smurf2 (NM 025481) | 5′–GATTCGACCAACAGCAACAGCAAG ttcaagagaCTTGCTGTTGCTGTTGGTCTTTTTTC–3′ |
1199–1217 |
a
Parentheses indicate GenBank™ accession numbers.
b
siRNAs are designated to encode two complementary sequences of 19 nucleotides homologous to a segment of Smurf1 or Smurf2 mRNA (underlined) separated by a nine-nucleotide space (small characters), and have a terminator signal (TTTTTTC) at a 3′ terminus.