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. 2007 Jan;4(1):9–17. doi: 10.1513/pats.200608-156JG

Figure 3.

Figure 3.

Schematic diagram for the work flow of 2D gel spot. Typically the proteins are in situ digested to peptides and the resulting peptide mixture is then analyzed on MALDI-TOF MS for PMF. After database search, if the protein is not identified or PTM is also needed, the peptide mixture will be analyzed on MALDI-TOF in the PSD mode, or the peptide mixture will be analyzed on an ion trap instrument or Q-TOF to get MS/MS spectra. After database search or de novo sequencing, the peptide can be identified and the PTM can be identified and located.