Figure 3.
(A) Matrix elastin yield within RASMC cultures supplemented with CuNPs and HA oligomers. The ratio of matrix elastin deposited to total elastin synthesized was calculated in each test case and was further normalized to similar ratio in control cultures. (B) LOX protein amounts in pooled medium aliquots collected over 21 days of culture. Shown are mean ± SD of DNA-normalized intensities, measured from representative SDS-PAGE/Western blots containing bands corresponding to LOX produced in the respective cases. (C) LOX enzyme activities in cultures treated with CuNPs and HA oligomers. Values (mean ± SD) are shown normalized to the LOX activity measured in control cell layers at 21 days of culture (n = 3 per case). * represents significance in differences relative to controls (P < 0.05).
