Table 1.
ELISA titersa |
||||||
---|---|---|---|---|---|---|
Immunogen | Alum-immunized group | c-di-GMP-immunized group | ||||
Total IgG | IgG1 | IgG2a | Total IgG | IgG1 | IgG2a | |
PdB | 30,000 | 40,000 | 8,000 | 60,000 | 40,000 | 25,000 |
(28,200 ± 3,250) | (54,200 ± 5,800) | |||||
PspA | 30,000 | 35,000 | 8,000 | 140,000 | 160,000 | 50,000 |
(24,700 ± 1,600) | (112,000 ± 17,200) | |||||
Alum | <500 | ND | ND | ND | ND | ND |
c-di-GMP | ND | ND | ND | <500 | ND | ND |
ELISA titers were determined as the reciprocal of the dilution of sera giving 50% of the highest absorbance reading above the background at 405nm. Values in parenthesis represent mean antibody titers ± SEM from six individual mice.
ND= Not Determined.