PHSCN inhibition of PHSRN peptide-induced angiogenesis in Matrigel plugs. (A) Angiogenesis induction by addition of PHSRN. X-axis, additions to Matrigel: PHSRN, Ac-PHSRN-NH2; HSPNR, Ac-HSPNR-NH2; bFGF, 400 µg of bFGF. Y-axis, mean number of microvessels per field (±SD). (B) Inhibition of PHSRN-induced angiogenesis by systemic PHSCN peptide treatment. X-axis, additions to Matrigel (Ac-PHSRN-NH2, Ac-HSPNR-NH2, or bFGF) and systemic treatments: PHSCN, Ac-PHSCN-NH2; HSPNC, Ac-HSPNC-NH2. Y-axis, mean number of microvessels per field (±SD), n = 30. (C) Examples of sectioned Matrigel plugs. Bars, 20 µm.