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. 2009 Mar;2(1):21–30. doi: 10.1593/tlo.08190

Figure 5.

Figure 5

Effects of SD on expression of cleaved caspase-3 in A431 cells. Cells were treated with SD (0–400 µM) for 48 hours, and then cells were collected by brief trypsinization. Total cell lysates were prepared and loaded to SDS-PAGE and Western blot analysis. Membranes were then probed with cleaved caspase-3 and β-actin antibodies followed by appropriate secondary antibody and ECL detection.