Fig. 3.

Immunostaining of AS-A in A. aegypti tissues. a Left protocerebral lobe of the brain. Neurons (n) with labeled processes (arrowhead) innervating the stomatogastric nervous system. Bent arrow indicates the front (arrowhead) and left side (arrow end) of the brain. b–d Abdominal ventral ganglia. Only one cell was stained in each ventral ganglion (vg), except in the last ganglion (d) in which eight cells were labeled. Note the immunostained varicosities in ventral processes (arrowheads). e, f Midgut. Endocrine cells (ec) showing immunoreactivity in the posterior midgut (mg) (mt Malpighian tubule). Two processes (arrowhead) can be seen running along the hindgut (hg) and ending in the rectal papillae (rp). Arrows Anterior part of the mosquito. b,c,e, f Combined images from confocal fluorescence (green) and light (yellow-red) microscopy. Bars 50 μm