Fig. 4.

Immunostaining of AS-A in A. albimanus tissues. a Right protocerebral lobe of the brain. Neurons (n) with labeled processes (arrowhead) innervating the stomatogastric nervous system. Bent arrow Front (arrowhead) and left side (arrow end) of the brain. b Processes (arrowhead) with varicosities projecting into the corpus cardiacum–corpus allatum (CC–CA) complex. c, d Abdominal ventral ganglia: only one cell was stained in each ventral ganglion (vg), except in the last ganglion (d) in which eight cells were labeled. Note the immunostained varicosities in ventral processes (arrowhead). d Oenocytes (eo) ventrally located in abdominal fat body showing immunoreactivity. e Midgut (mt Malpighian tubule). Endocrine cells (ec) showing immunoreactivity in the posterior midgut (mg). Note the two processes (arrowhead) running along the hindgut (hg) and ending in the rectal papillae. f Heart. Inmmunolabeled varicosities in processes (arrowhead) ending in the proximity of pericardial cells (pc) around the dorsal vessel (dv). Arrows Anterior part of the mosquito. c, e Combined images from confocal fluorescence (green) and light (yellow-red) microscopy. Bars 50 μm