Figure 4.

In vivo protease signals from tumors of various sizes either plotted with or normalized to corresponding vascular signals. Raw mean ProSense (A) and MMPSense (C) along with AngioSense signals for each tumor. As seen, protease expressions remain rather constant and are statistically independent of tumor size, which is not caused by a lack of available protease probe at the cancerous region as evident by AngioSense signals. Mean ProSense (B) and MMPSense (D) expressions normalized to respective biodistribution signals (AngioSense) for tumors of different categorical areas. This internal control provides a statistically relevant way to observe disease progression.