Figure 1.

Lung cancer cells produced high amounts of MCP-1 and IL-8. The cytokines' production in CM collected from lung cancer cells was measured using a human cytokine antibody array. (A) Images of the array that were assayed with CM obtained from control media, BEAS2B, H1299, and A549 cells. Details of the procedure are described in Materials and Methods. The signals of MCP-1, RANTES, ENA-78, GRO, GROα, IL-8, VEGF, CXCL16, MMP-9, and TIMP-4 were labeled with rectangles. (B) Radiographs of the arrays were scanned to determine the density of each cytokine. Data are presented as a mean value of fold increase relative to control media. The value for each scan was adjusted based on the intensity of positive control spots on each membrane.